Blood And Tissues

TITLE: BLOOD & TISSUES- Staining Of Blood Films & Identification Of Leukocytes. OBJECTIVES: i.To estimate the preparation of short letter films from human blood sample. ii.To learn the techniques of discolor the blood films with Giemsa filthiness and Wright territory. iii.To identify and make drawing of leukocytes that bribe in human blood sample under microscope. interpellation: Leukocytes be divided into granulocytes and agranulocytes. Granulocytes be also known as polymorphonuclear leukocytes, or polys for short. Three types of granulocyte are neutrophils, bromeosinophils, basophils. Meanwhile, there are ii types of agranulocyctes which are lymphocytes and monocytes. If we observe the smear as it is after fixing, we turn over on arrest really little because all cells are very transparent. The erythrocytes are slightly visible, but the leukocytes are excessively pale, just about invisible and we will not see anything inside them. To be able to obser ve and recognize the diverse kinds of leukocyte, we must dirt them. For this purpose, normally Giemsa turd and Wright stain are used. Giemsa stain is a mixture of dyes, based on methylene blue (basic dye) and eosin ( acidic dye). Giemsa stain is a classical blood film stain for peripheral blood smears and bone pump specimens. The principle of Giemsa stain is the basic dye stained the acidic sectionalisation of the burden of the cell and the acidic dye stained the basic part of the cytoplasm of the cell. Therefore, the Giemsa stain allows clear observations to discern types of leukocytes. By patch of the cells we can observe the different types of unobjectionable blood cells (leukocyctes) which are, neutrophils, eosinophils, basophils, lymphocytes and monocytes under the light microscope. MATERIALS: 1)Alcohol swabs 2)Blood Lancets 3)Deionized water 4)Distilled water 5)Giemsa stain 6)Glass Slides 7)Jar of disinfection for disposal of slides 8)Methanol 9)Microsc opes 10)Oil Immersion 11)Pasteur pipettes ! 12)Sorensens...If you want to get a total essay, fix it on our website: OrderCustomPaper.com

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